A primary reaction to stress induced by high copper ion concentration
Bozhkov A.I., Goltvyanskij A.V., Rostama Sh.- V.N. Karazin National University, Research Institute of Biology
- 4, Sq. Svobody, 61077 Kharkov, Ukraine
The dynamics of cell aggregates forming in Dunaliella viridis Teod. were studied. Two cultures of D. viridis, sensitive to CuSO4 (CuS) and resistant to CuSO4 (CuR), were studied. Almost immediately after the addition of CuSO4 to the culture medium the cells became immobile and formed cell aggregates. The manifestation of these changes depends on the cell culture functional state. Cell recovery after CuSo4 treatment was investigated. In CuR-culture cell aggregates become separated and cell mobility and cell membrane integrity recovered relatively early. It is demonstrated that cell aggregation is a primary reaction of cells to high concentration of copper ions in the cell culture medium.
Keywords: Dunaliella viridis, copper sulfate, cell aggregates
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