The tetrasol method (MTT-test) for assessment of metabolic activity of cells of fucoid algae was modificated. Estimation of optimum conditions both sample preparation and reaction passing: concentration of MTT solution, temperature, time of interaction with reagent was carried out. A possibility of the MTT-test usage for an estimation of influence of environmental factors (including oil pollution) on a seaweeds is shown.

Keywords: Fucus vesiculosus, MTT (3-[4, 5-dimetiltiasol-2-il]-2, 5-dipheniltetrasolia bromide), metabolic activity of cells (MAC), salinity, oil pollution

Full text: PDF 176K

1. Back S., C. Collins, and G. Russell., Brit. Phycol. J., 27:39–47, 1992. http://dx.doi.org/10.1080/00071619200650061
2. Chang Wan-Chun, Ming-Hwa Chen, and Tse-Min Lee, Bot. Bull. Acad. Sin., 40:207–212, 1999.
3. Coelho S.M., Jan W., Rijstenbil, and Murray T., J. Aquat. Ecosyst. Stress Rec., 7:317–333, 2000. http://dx.doi.org/10.1023/A:1009916129009
4. Drobyshev V.P., Ekologiya, 1:96–98, 1971.
5. Drobyshev V.P., Tsitologiya, 12(7):97–102, 1970.
6. Huang Y., Wang J., Li G., Zheng Z., and Su W., FEMS Immunol. Med. Microbiol., 31:163–167, 2001. http://dx.doi.org/10.1111/j.1574-695X.2001.tb00513.x
7. Kalina M. and Palmer J.M., Histochemie, 14:366–374, 1968. http://dx.doi.org/10.1007/BF00304260
8. Karsten U., Phycol. Res., 55:257–262, 2007. http://dx.doi.org/10.1111/j.1440-1835.2007.00468.x
9. Karsten U., Wiencke C., and Kirst G., J. Plant Physiol., 138:667–673, 1991. http://dx.doi.org/10.1016/S0176-1617(11)81313-1
10. Lu I-Fan, Ming-Shiuan Sung, and Tse-Min Lee, Mar. Biol., 150(1):1–15, 2006.
11. Lüning K., J. Phycol., 30:193–199, 1994. http://dx.doi.org/10.1111/j.0022-3646.1994.00193.x
12. Lure Yu.Yu., Unifitsirovannye metody analiza vod. 2-e izd., Khimiya, Moskva, 1973. [Rus.]
13. Makarov V.N., Schoschina E.V., and Lüning K., Eur. J. Phycol., 30:261–266, 1995. http://dx.doi.org/10.1080/09670269500651031
14. Malavenda S.V., Vliyanie solenosti na fukusovye vodorosli Barentseva morya: Avtoref. dis. … kand. biol. nauk, Murmansk, 2007. [Rus.]
15. Malm T., Kautsky L., and Enkvist R., Bot. Mar., 44:101–108, 2001. http://dx.doi.org/10.1515/BOT.2001.014
16. Nam Bo-Hye, Jin Hyung-Joo, Kim Se-Kwon, and Hong Yong-Ki, J. Appl. Phycol., 10:31–36, 1998. http://dx.doi.org/10.1023/A:1008059314639
17. Roche Applied Science. Apoptosis, Cell Death and Cell Proliferation, Manual, Mannheim, 2004.
18. Schrader K.K. and Harries M.D., Aquacult. Res., 37:928–937, 2006. http://dx.doi.org/10.1111/j.1365-2109.2006.01514.x
19. Serrao E.A., Kautsky L., and Brawley S.H., Oecologia, 107:1–12, 1996. http://dx.doi.org/10.1007/BF00582229
20. Stepanyan O.V. and Voskoboynikov G.M., Biol. Morya, 32(4):241–248, 2006.
21. Towill L.E. and Mazur P., Can. J. Bot., 53:1097–1102, 1975. http://dx.doi.org/10.1139/b75-129
22. Tropin I.V., Radzinskaya N.V., and Voskoboynikov G.M., Izv. RAN. Ser. Biol., 1:48–56, 2003.
23. Vistica D., Skehan Ph., Scudiero D., Monks A., Pittman A., and Boyd M., Canc. Res., 51:2515–2520, 1991.