The qualitative and quantitative structure of the diatom microalgae Phaeodactylum tricornutum carotenoids, cultivated under the different temperature and illumination was investigated. It was stated, that carotenoid composition of the microalgae is represented by 5 fractions: ß-carotene, fucoxanthine, diadinoxanthine diatoxanthine and diadinochrome. Fucoxanthine is located in two-isomeric forms: trans- and cis-. It was stated, that carotenoids accumulation depended on the cultivating conditions. The maximal fucoxanthene accumulation occurs under the temperature 30°C and constant illumination 4.5 klx.

Keywords: microalgae Phaeodactylum tricornutum, carotenoids, fucoxanthin, temperature, light

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