ISSN (print) 0868-8540, (online) 2413-5984
logoAlgologia
  • 3 of 7
Up
Algologia 2014, 24(1): 34–45
https://doi.org/10.15407/alg24.01.034
Physiology, Biochemistry, Biophysics

Production characteristics of the microalgae Porphyridium purpureum (Bory) Ross. under batch and semicontinuous cultivation

Gudvilovich I.N., Borovkov A.B.
Abstract

It is shown that cultivation of Porphyridium purpureum using semicontinuous method for optimal selection of key parameters allows to obtain stably high productivity of this species both by biomass, and by its a valuable components in comparison with other modes. Production characteristics of semicontinuous culture P. purpureum has been defined. Productivity of P. purpureum increases with the growth of specific flow rate of the medium. The highest productivity of biomass and pigments is realized in the range of flow rate of the medium 0.3–0.4 day-1, and reaches: of biomass – 0.5 g AFDW · l-1 · day-1 and of B-phycoerythrin – 40 mg · l-1 · day-1. The type of change of the pigments content P. purpureum has been determined under semicontinuous cultivation; the possibility of regulation of pigments content with the help of varying the specific flow rate has been shown. The relative content of pigments in the biomass of P. purpureum in the range of specific flow rate of the medium 0.1–0.4 day-1 increases by 50 %. The maximum pigment content in the biomass of P. purpureum is noted at the specific flow rate 0.3–0.4 day-1. Productivity of semicontinuous culture P. purpureum by biomass and pigments is 1.5–3 times higher than its productivity by batch cultivation, which is confirmed by experimental data.

Keywords: Porphyridium purpureum, batch culture, semicontinuous culture, the density of the culture, phycobiliproteins, productivity

Full text: PDF (Rus) 496K

References
  1. Abd El-Baky H. J. Med. Sci., 3(4):314–324, 2003.http://dx.doi.org/10.3923/jms.2003.314.324
  2. Algarra P. and Ruediger W., Plant. Cell. Environ, 16(2):149–159, 1993.http://dx.doi.org/10.1111/j.1365-3040.1993.tb00856.x
  3. Borowitzka M.A., J. Appl. Algol., 7: 3–15, 1995.
  4. Borovkov A.B., Dinamika pigmentov i rosta mikrovodoroslej v hemostate na primere Dunaliella salina Teod., Avtoref. dis. … kand. biol. nauk,Sevastopol', 2008. [Rus.]
  5. Drobeckaja I.V. Vlijanie uslovij mineral'nogo pitanija na rost i himicheskij sostav Spirulina platensis (Nordst.) Geitler, Avtoref. dis. … kand. biol. nauk, Sevastopol', 2005. [Rus.]
  6. Efremova N. Razrabotka sposobov poluchenija antioksidantnyh preparatov na osnove bioaktivnyh veshhestv cianobakterij i mikrovodoroslej, Avtoref. dis. … d-ra biol. nauk, Kishinjov, 2009. [Rus.]
  7. Finenko Z.Z., Churilova T.Ja., and Akimov A.I., Mikrovodorosli Chjornogo morja: problemy sohranenija bioraznoobrazija i biotehnologicheskogo ispol'zovanija, Sevastopol', Pp. 301–319, 2008.
  8. Fabregas J., Garcia D., Morales E. et al., J. Ferment. Bioeng., 86(5):477–481, 1998.http://dx.doi.org/10.1016/S0922-338X(98)80155-4
  9. Fernandez A.F.G., Camacho G.F., Perez S.J.A. et al., Biotechnol. Bioeng., 58:605–616, 1998.http://dx.doi.org/10.1002/(SICI)1097-0290(19980620)58:6<605::AID-BIT6>3.0.CO;2-M
  10. Glazer A. N. and Hixson C.S., J. Biol. Chem., 252(1):32–42, 1977.
  11. Grossman A.M., Schaefer R., Chiang G.G., and Collier J.L., Molecular biology of cyanobacteria, Acad. Publ., Kluwer, Pp. 641–675, 1994.http://dx.doi.org/10.1007/978-94-011-0227-8_21
  12. Gudvilovich I.N., Ekol. Morja, 81: 28–36, 2010.
  13. Hirata T., Tanaka M., Ooike M. et al. J. Appl. Phycol., 12(3):435–439, 2000.http://dx.doi.org/10.1023/A:1008175217194
  14. Jahn W., Steinbiss J., and Zetsche K., Planta, 16(6):536–539, 1984.http://dx.doi.org/10.1007/BF00407086
  15. Kathiresan S., Sarada R., Bhattacharya S., and Ravishankar A., Biotech. and Bioeng., 96:456–463, 2006.http://dx.doi.org/10.1002/bit.21138
  16. Kopecky J., Riederer M., Pfuendel E., Arch. Hydrobiol. (Suppl.) (Algol. Stud.), 142:189–195, 2002.
  17. Lelekov A.S., Modelirovanie rosta i biosinteza morskih mikrovodoroslej v kvazinepreryvnoj kul'ture, Avtoref. dis. … kand. biol. nauk, Sevastopol', 2009. [Rus.]
  18. Metody fiziologo-biohimicheskogo issledovanija vodoroslej v gidrobiologicheskoj praktike, Nauk. dumka, Kiev, 1975. [Rus.]
  19. Minjuk G.S., Drobeckaja I.V., Chubchikova I.N., and Terent'eva N.V., Mor. ekol. J., 7(2):5–23, 2008.
  20. Singh S., Arad S., and Richmond A., J. Appl. Phycol., 12:269–275, 2000.http://dx.doi.org/10.1023/A:1008177002226
  21. Stadnichuk I.N., Fikobiliproteiny. Biologicheskaja himija, Mir, Moscow, 1990. [Rus.]
  22. Sud'ïna O.G., Shnjukova Je.I., Mushak P.O., Los' S.I. ta in., Biohimija chervonih vodorostej, Kyiv., 2007. [Ukr.]
  23. Trenkenshu R.P., Rostovye i fotojenergeticheskie harakteristiki morskih mikrovodoroslej v plotnoj kul'ture, Avtoref. dis. … kand. biol. nauk, Krasnojarsk, 1984. [Rus.]
  24. Trenkenshu R.P., Beljanin V.N., Biol. morja, 51:41–46, 1979.
  25. Uil'jams U. Dzh., Opredelenie anionov, Himija, Moscow, Pp. 134–136, 1982. [Rus.]
  26. Upitis V.V., Pakalne D.S., and Shulce I.F., Izv. AN Latv. SSR, 505(8):95–104, 1989.
  27. Xiao H., Xie Z., and Guo J., J. Fujian Teach. Univ.,17(2):78–80, 2001.
  28. Yamanaka G. and Glazer A.N., Arch. Microbiol., 124:39–47, 1980.http://dx.doi.org/10.1007/BF00407026