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Algologia 2014, 24(2): 147–162
https://doi.org/10.15407/alg24.02.147
Physiology, Biochemistry, Biophysics

Functional heterogeneity of microalgal cells Desmodesmus communis (E. Hegew.) E. Hegew. (Chlorophyta) under normal and toxic exposure

Dmitrieva A.G., Filenko O.F., Ipatova V.I.
Abstract

The functional heterogeneity of microalgal cells (Desmodesmus communis (= Scenedesmus quadricauda (Turp.) Brèb.) was studied at different stages of development under normal and toxic exposure using the method of microcultures that enables to check development of single cells in small volume within 80 hours with a daily view of the state and the number of cells. The method allows to estimate the ratio of dividing, dormant and dead cells under normal and toxic exposure. Toxicants (CuCl2, AgNO3, colloidal silver) at different concentrations were added directly to makroculture at different stages of its development: the lag-, logarithmic and stationary growth phases. The proportion of dormant cells in normal makroculture was elevated in the lag phase at the beginning of the development of culture and the stationary phase, while the proportion of dying cells remained more or less constant. The number of dormant cells during growth of the culture varied depending on the toxicant, and the phase of the development of culture. After long periods of repeated intoxication in an increasingly toxic load and subsequent reinoculation of culture in a clean media, the ratio of dividing, dormant and dead cells over time was close to what took place after the initial intoxication. Inhibition of cell division is one of the main factors of toxicity, which depends on the physiological state of the culture and the level of toxicity. The rate of cell division at different stages of culture may be increased at low levels of the toxic exposure.

Keywords: algae, population heterogeneity, copper chloride, nitrate of silver, colloidal silver

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