Optimization of the spectral composition of photosynthetically active light sources is highly relevant in the biotechnological production of commercially valuable species of algae, particularly blue-green algae Spirulina platensis. The aim of the research was to study the functioning of the antioxidant system of S. platensis algae cultivated under LED light sources with different spectral composition. Analysis of changes of productivity, as well as chlorophyll, phycocyanin, carotenoids, tocopherols, glutathione amounts and activity of ascorbate peroxidase, catalase and glutathione reductase in the cells of S. platensis grown under LED lighting has been done. The effect of LED lighting with different spectral composition on the state of the main components of the antioxidant system of S. platensis has been studied. Reduction of the antioxidant status of cells of the S. platensis algae cultivated under LED illumination with various combinations of red, yellow and blue LEDs is revealed. It is shown that, when using blue LEDs as a source of photosynthetically active light the growth of S. platensis is inhibited. It is revealed that under the conditions of an excess of red light in spite of the substantial (by 30%) increase in the productivity of S. platensis, decrease in phycocyanin content in cells in relation to other pigments takes place. Application of an illuminator with yellow and two blue spectral bands in addition to the red light leads to increase in the productivity of S. platensis by 17% compared to white light, as well as increase in production of valuable antioxidant phycocyanin in algae cells by more than 15%. It was found that the samples with increased productivity, grown under the red, combined red and blue light, and under illuminator emitting red, yellow and two types of blue light, had decreased amount of low-molecular antioxidant tocopherol, as well as lower activities of antioxidant enzymes ascorbate peroxidase and catalase, while application only blue light for growing S. platensis causes a decrease in the amount of reduced glutathione and activity of the glutathione reductase.

Keywords: Spirulina platensis, antioxidant system, photosynthetically active light, the spectral composition of the LEDs

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