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Algologia 2016, 26(2): 152–162
https://doi.org/10.15407/alg26.02.152
Ecology, Cenology, Conservation of Algae and Their Role in Nature

Activity of antioxidant enzymes of cyanoprokaryota and green microalgae when culturing under different temperature conditions

Kureishevich A.V. 1, Nezbrytskaya I.N. 1, Stanislavchuk A.V. 2
Abstract

The effect of different temperature conditions of cultivation (20, 26 and 32 °C) on the activity of antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathioneperoxidase (GPO), of some species of Cyanoprokaryota (Aphanocapsa planctonica (G.M. Sm.) Komárek et Anagn., Phormidium autumnale (C. Agardh) Gomont f. uncinata (C. Agardh) N.V. Kondrat.) and Chlorophyta (Desmodesmus communis (E. Hegew.) E. Hegew., Tetraedron caudatum (Corda) Hansg.). was studied. The activity of antioxidant enzymes depends on the temperature, culture age and species affiliation. At the maximal temperature (32 °C), as compared to 20 and 26 °C, the activity of SOD, CAT and GAP in Cyanoprokaryota and green microalgae was significantly inhibited, especially in older cultures. It has been shown that under the studied temperatures the peake of SOD and CAT activity of both representatives of Cyanoprokaryota and Chlorophyta do not coincide, and mainly are in the antiphase. At 20 and 26 °C in older cultures of Cyanoprokaryota and Chlorophyta the enzymatic activity of GAP is significantly higher as compared to younger ones. At the same time, at the maximal temperature (32 °C) its enzymatic activity was almost completely inhibited.

Keywords: Cyanoprokaryota, Chlorophyta, temperature, antioxidant enzymes superoxide dismutase, catalase, glutathioneperoxidase

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