ISSN (print) 0868-8540, (online) 2413-5984
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Algologia 2020, 30(1): 19–26
Ecology, Cenology, Conservation of Algae and Their Role in Nature

Chlorophyta growth rate on different cultivation media

Leontieva T.A., Kirpenko N.I.

The growth rates (GR) of green microalgae cultures of the genera Desmodesmus (Chodat) S.S.An, T.Friedl & E.Hegewald, Scenedesmus Meyen, Acutodesmus (Hegewald) Tsarenko, and Selenastrum Reinsch were studied. Algologically pure cultures of microalgae were grown in batch culture at a temperature of 25 ± 0.5 °С, illumination 2500 lx with alternating light: darkness 16 : 8 h, on various cultural media (Fitzgerald in modification of Zehnder and Gorham, Tamiya and Bold), which differ significantly in content of basic nutrient elements: nitrogen and phosphorus. Microscopic analysis of cultures during cultivation showed noticeable differences in the GR of the studied species of microalgae under these conditions. The highest GR values were recorded on Fitzgerald medium with representatives of the genus Scenedesmus leading in GR values. At the same time, species-specific differences were observed within the studied genera. For example, the GR of Scenedesmus ellipticus Corda was higher than S. obtusus Meyen, and Desmodesmus subspicatus (Chodat) E.Hegewald & A.Schmidt had higher GR compared to D. communis (E.Hegewald) E.Hegewald. On the Bold medium, as well as on the Fitzgerald medium, S. ellipticus was characterized by the highest GR, while on the Tamiya medium it had very low rates. The Tamiya medium turned was the least favorable for growth of the studied microalgae species, with the exception of D. subspicatus; its number exceeded those of other species. Depending on the nutrient medium, the dynamics of culture growth also changed, in particular, the duration of the development phases.

Keywords: Chlorophyta, growth rate, nutrient media

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