ISSN (print) 0868-8540, (online) 2413-5984
logoAlgologia
  • 1 of 8
Up
Algologia 2020, 30(1): 3–18
https://doi.org/10.15407/alg30.01.003
Physiology, Biochemistry, Biophysics

Preliminary adaptation of Dunaliella viridis strains to copper sulfate affects the thermal stability of the culture

Bozhkov A.I., Kovalоva M.K., Goltvianskiy A. V., Ushakova E.O., Tsapko H.Ye., Gavrish А.O.
Abstract

We studied the growth of copper-sensitive (CuS D. v.) and copper-resistant (CuR D. v. 75) strains of the green microalga Dunaliella viridis Teodoresco at 35 oС to determine the relationship between the induced resistance to copper ions and resistance to high temperature of the environment. The effect of stepwise temperature increasing from 24 → 29 → 35 °C with an interval of 7 days on the growth rate and biomass composition (content of DNA, RNA, protein, triacylglycerides (TG), carotenoids and chlorophyll) of CuS D. v. and CuR D. v. cultures was examined. It was revealed that a temperature increase of up to 35° in the culture of CuS D. v. at the initial stage of growth slows its growth; the culture CuR D. v. 75 dies under the same conditions. With a stepwise increase in the temperature of cultivation (24 → 29 → 35 °C), the culture CuR D. v. 75 survives, its growth rate is slightly higher than in CuS D. v. proving the thermal stability of its cells. In addition, biomass of CuR D. v. 75 contains more protein, DNA, TG, and especially β-carotene, compared to CuS D. v. At a temperature of 35 °C, the content of protein, DNA, TG, and β-carotene in cells of CuS D. v. also increased. It has been found that there is a complex relationship between resistance to copper ions and resistance to high temperature, which is determined by the temporal nature of the temperature change.

Keywords: copper ion resistance, epigentotype, metabolism, Dunaliella viridis, high temperature

Full text: PDF (Rus) 653K

References
  1. Bagheri M., Mansouri H. 2014. Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L. Appl. Biochem. and Biotechnol. 175(5): 2366–2375. https://doi.org/10.1007/s12010-014-1435-8 https://www.ncbi.nlm.nih.gov/pubmed/25492688
  2. Barati B., Lim P.E., Gan S.Y., Sze-Wan Poong, Siew-Moi Phang, Beardall J. 2018. Effect of elevated temperature on the physiological responses of marine Chlorella strains from different latitudes. J. Appl. Phycol. 30(1): 1–13. https://doi.org/10.1007/s10811-017-1198-z
  3. Bozhkov A.I., Goltvianskiy A.V. 1998. Induction of resistance to copper sulfate in Dunaliella viridis Teod. Algologia. 8(2): 162–169.
  4. Bozhkov A.I., Menzyanova N.G. 1997. Age dependence of lipid metabolism and beta-carotene content in cells of Dunaliella viridis Teod. Hydrobiol. J. 33(6): 132–138.
  5. Bozhkov A.I., Menzyanova N.G., Kovalova M.K. 2008. Annual rhythm of growth intensity of microalgal culture Dunaliella viridis Teod. (Chlorophyta) and fluctuations of some heliophysical factors. Int. J. Algae. 10(4): 350–364. https://doi.org/10.1615/InterJAlgae.v10.i4.50
  6. Bozhkov A.I., Menzyanova N.G., Kovalova M.K. 2009. Seasonal peculiarities of the epigenotype formation in the copper-sensitive and copper-resistant strain of Dunaliella viridis Teod. in the process of accumulative cultivation. Int. J. Algae. 11(2): 128–140. https://doi.org/10.1615/InterJAlgae.v11.i2.30
  7. Bozhkov A.I., Goltvianskiy A.V., Kovalova M.K., Menzyanova N.G. 2018. On the inheritance of induced resistance to toxic concentrations of sulfur acid of copper by subsequent cell generations of Dunaliella viridis Teod. Alglogia. 28(4): 387–408. https://doi.org/10.15407/alg28.04.387
  8. Bozhkov A.I., Menzyanova N.G., Sedova K.V., Goltvianskiy A.V. 2010. The effect of high temperature on cell sensitive and resistant to copper ions Dunaliella viridis Teod. (Chlorophyta). Algologia. 20(4): 413–431.
  9. Camejo D., Rodríguez P., Morales M.A., Dell'Amico J.M., Torrecillas A., Alarcón J.J. 2005. High temperature effects on photosynthetic activity of two tomato cultivars with different heat susceptibility. J. Plant Physiol. 162(3): 281–228. https://doi.org/10.1016/j.jplph.2004.07.014 https://www.ncbi.nlm.nih.gov/pubmed/15832680
  10. Davis R.W., Carvalho B.J., Jones H.D.T., Singh S. 2015. The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis. J. Appl. Phycol. 27: 109–123. https://doi.org/10.1007/s10811-014-0331-5 https://www.ncbi.nlm.nih.gov/pubmed/25620852 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4297879
  11. Dere Ş., Güneş T., Sivaci R. 1998. Spectrophotometric determination of Chlorophyll-A, B and total carotenoid contents of some algae species using different solvents. Tr. J. Bot. 22: 13–17.
  12. Fachet M., Hermsdorf D., Rihko-Struckmann L., Sundmacher K. 2016. Flow cytometry enables dynamic tracking of algal stress response: A case study using carotenogenesis in Dunaliella salina. Algal Res. 13: 227–234. https://doi.org/10.1016/j.algal.2015.11.014
  13. Grama B.S., Agathos S.N., Jeffryes C.S. 2016. Balancing photosynthesis and respiration increases microalgal biomass productivity during photoheterotrophy on glycerol. ACS Sustanable Chem. Eng. 4(3): 1611–1618. https://doi.org/10.1021/acssuschemeng.5b01544
  14. Gubler E.V., Genkin A.A. 1973. Application of non-parametric statistical criteria in biomedical research. Leningrad: Medicine. 141 р. [Rus.]
  15. Jian-Kang Zhu. 2016. Abiotic Stress Signaling and Responses in Plants. Сell. 167(2): 313–324. https://doi.org/10.1016/j.cell.2016.08.029 https://www.ncbi.nlm.nih.gov/pubmed/27716505 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5104190
  16. Kovalova M.K., Menzyanova N.G., Jain A., Yadav A., Flora S.J.S., Bozhkov A.I. 2012. Effect of hormesis in Dunaliella viridis Тeodor. (Chlorophyta) under the influence of copper sulfate. Int. J. Algae. 14(1): 44–61. https://doi.org/10.1615/InterJAlgae.v14.i1.40
  17. Lowry О.B., Rosebrough N.J., Farr A.L., Randall B.J. 1957. Protein measurement with Folin phenol reagent. Biol. Chem. 93: 265–273.
  18. Nezhad F.S., Mansouri H. 2017. Effects of polyploidy on response of Dunaliella salina to salinity. bioRxiv. 15: 1-29.
  19. Prasch Ch.M., Sonnewald U. 2015. Signaling events in plants: Stress factors in combination change the picture. Environ. and Exp. Bot. 114: 4–14. https://doi.org/10.1016/j.envexpbot.2014.06.020
  20. Schmalhausen I.I. 1946. Evolution factors (stabilizing selection theory). Moscow: Polygraph Book. 396 р. [Rus.]
  21. Singh P., Baranwal M., Reddy S.M. 2016. Antioxidant and cytotoxic activity of carotenes produced by Dunaliella salina under stress. Pharm. Biol. 54(10): 2269–2275. https://doi.org/10.3109/13880209.2016.1153660 https://www.ncbi.nlm.nih.gov/pubmed/26983781
  22. Spirin A.S. 1958. Spectrophotometric determination of the total amount of nucleic acids. Biochemistry. 23: 656-662.
  23. Sutherland D.L., Howard-Williams C., Turnbull M.H., Broady P.A., Craggs R.J. 2015. Enhancing microalgal photosynthesis and productivity in wastewater treatment high rate algal ponds for biofuel production. Biores. Technol. 184: 222–229. https://doi.org/10.1016/j.biortech.2014.10.074 https://www.ncbi.nlm.nih.gov/pubmed/25453429
  24. Yeon A., You S., Kim M., Gupta A., Park M.H., Weisenberger D.J., Liang G., Kim J. 2018. Rewiring of cisplatin-resistant bladder cancer cells through epigenetic regulation of genes involved in amino acid metabolism. Theranostics. 8(16): 4520–4534. https://doi.org/10.7150/thno.25130 https://www.ncbi.nlm.nih.gov/pubmed/30214636 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134931