Comparative analysis of methods for assessing the safety of Dunaliella salina Teodoresco and Chlorococcum dissectum Korshikov (Chlorophyta) microalgae cultures after exposure to stress factors

Authors

Chernobai N.A.*, Vozovik K.D., Kadnikova N.G.
Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, 23 Pereyaslavska Str., Kharkiv 61016, Ukraine
* nadiiachernobai@gmail.com

Section:

Physiology, Biochemistry. Biophysics

Issue:

Vol. 31 No. 4 (2021)

Pages:

353–364

DOI:

https://doi.org/10.15407/alg31.04.353

Abstract

The possibility of using various methods for determining the viability of cultures of microalgae Dunaliella salina and Chlorococcum dissectum before and after freezing-warming was investigated and analyzed. It has been established that the selection of an effective method should be carried out individually for each culture. For an integral assessment of the proliferative and metabolic activity of cells of both species of the studied microalgae, Alamar Blue-test and the ability to grow on liquid nutrient media can be used. The use of the Koch plate method, MTT-test and TTC staining is possible only for the microalga C. dissectum. Vital staining with trypan blue was found to be incorrect.

Keywords:

viability, microalgae, Chlorococcum dissectum, Dunaliella salina, Alamar Blue, MTT-test, TTC, trypan blue

Full text

References

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Citation

Chernobai N.A., Vozovik K.D., Kadnikova N.G. 2021. Comparative analysis of methods for assessing the safety of Dunaliella salina Teodoresco and Chlorococcum dissectum Korshikov (Chlorophyta) microalgae cultures after exposure to stress factors. Algologia. 31(4): 353–364. https://doi.org/10.15407/alg31.04.353