The protein synthesis activity and protein content, the content of the different types of nucleic acids and lipids in cells of microalgae Dunaliella viridis Teod., copper-sensitive culture (CuS-culture) and copper resistant culture (CuR-culture), were researched. It was found, that formation of cell resistance to high copper concentration was accompanied by the formation different from the control culture epigenotype, which was characterized by: a) decreasing total RNA and ribosomal RNA content; b) decreasing protein synthesis activity and protein content; c) decreasing protein 70 kDa fraction and increasing protein 35 kDa fraction; d) increasing lipid content in cytosol (3-10 times for different lipid fraction). It is shown that cells of CuR-culture D. viridis have increased resistance to short-term effects of high temperature (45 °C for 1.5–2.0 min) compared with the of CuS-culture. Increased thermoresistance CuR-cultures of D. viridis correlated not only with decreasing content of ribosomes and protein, but with a high content of copper ions in cells.

Keywords: proteins, lipids, nucleic acids, resistance, temperature, Dunaliella viridis

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