Green algae (Chlorophyta) cultures are potentially valuable for biotechnology
Authors
,
Panyuta О.О.,
Belava V.N.
Section:
Physiology, Biochemistry. BiophysicsIssue:
Vol. 36 No. 2 (2026)Pages:
67-87DOI:
https://doi.org/10.15407/alg36.02.067Abstract
A comparative assessment in four species of green microalgae (Ankistrodesmus braunii (Nägeli) Lemmerm, Chlamidomonas reinhardii Dang, Chlorella vulgaris Beijer, and Halochlorella rubescens P.J.L.Dang) for protein, amino acids, chlorophylls a and b, carotenoids, sulfolipids, and acylcarnitines content was carried out. The content of potentially valuable for biotechnology substances was: Proteins ― 19.17–28.33 mg/g dry weight (DW); Amino acids ― 174.4–374.32 mkM/g DW; Essential amino acids ― 34.88–77.52 mkM/g DW; Acylcarnitines ― 0.91–1.62 mkM/g DW; Sulfolipid ― 0.8–3.83 mg/g DW; Chlorophylls a and b ― 5.86–24.57 mg/g DW; Carotenoids ― 1.18–2.4 mg/g DW. The amino acid content was: A. braunii ― 374.32 mkM/g DW, S. rubescens and C. vulgaris ― 289.22 and 274.97 mkM/g DW, respectively, C. reinhardii ― 174.4 mkM/g DW. The content of five essential amino acids (valine, leucine, methionine, tryptophan, phenylalanine) was highest in S. rubescens (77.52 mkM/g DW), 1.5 times lower in A. braunii, 1.9 times lower in C. vulgaris, and 2.2 times lower in C. reinhardii. The total content of 15 acylcarnitines was approximately 1.5 times higher in A. braunii and C. vulgaris than in C. reinhardii and S. rubescens. The sulfolipid content was highest in A. braunii (3.83 mg/g DW), almost two times lower in C. vulgaris, and 4.5 times lower in C. reinhardii and S. rubescens. The total content of chlorophylls a and b was highest in C. reinhardii (24.57 mg/g DW), and the carotenoid content was highest in A. braunii (2.4 mg/g DW).
Keywords:
green microalgae, algae biotechnology, amino acids, protein, chlorophyll, carotenoids, sulfolipid, acylcarnitinesFull text
References
Abu-Ghosh S., Fixler D., Dubinsky Z., Solovchenko A., Zigman M., Yehoshua Y., Ilu D. 2015. Flashing light enhancement of photosynthesis and growth occurs when photochemistry and photoprotection are balanced in Dunaliella salina. European Journal of Phycology. 50(4): 469–480. https://doi.org/10.1080/09670262.2015.1069404
Acquah C., Tibbetts S.M., Pan S., Udenigwe C. 2020. Nutritional quality and bioactive properties of proteins and peptides from microalgae. In: Handbook of Microalgae-Based Processes and Products. Cambridge: Acad. Press. Pp. 493–531. https://doi.org/10.1016/B978-0-12-818536-0.00019-1
Algae: Reference Book. 1989. Ed. S.P. Wasser. Kyiv: Nauk. Dumka. 608 p. [Водоросли: Справочник. Под ред. С.П. Вассера. Киев: Наук. думка. 608 с.]
Becker E.W. 2007. Microalgae as a source of protein. Biotechnology Advances. 25(2): 207–210. https://doi.org/10.1016/j.biotechadv.2006.11.002 https://www.ncbi.nlm.nih.gov/pubmed/17196357
Beheshtipour H., Mortazavian A.M., Mohammadi R., Sohrabvandi S., Koshravi-Darani K. 2013. Supplementation of Spirulina platensis and Chlorella vulgaris algae into probiotic fermented milks. Comprehensive Reviews in Food Science and Food Safety. 12: 144–154. https://doi.org/10.1111/1541-4337.12004
Bermejo E., Filali R., Behnam T. 2021. Microalgae culture quality indicators: a review. Critical Reviews in Biotechnology. 41(4): 457–473. https://doi.org/10.1080/07388551.2020.1854672 https://www.ncbi.nlm.nih.gov/pubmed/33653198
Bourdin B., Adenier H., Perrin Y. 2007. Carnitine is associated with fatty acid metabolism in plants. Plant Physiology and Biochemistry. 45(12): 926–931. https://doi.org/10.1016/j.plaphy.2007.09.009 https://www.ncbi.nlm.nih.gov/pubmed/17988884
Breuer G., Lamers P.P., Martens D.E., Draaisma R.B., Wijffels R.H. 2013. Effect of light intensity, pH, and temperature on triacylglycerol (TAG) accumulation induced by nitrogen starvation in Scenedesmus obliquus. Bioresource Technology. 143: 1–9. https://doi.org/10.1016/j.biortech.2013.05.105 https://www.ncbi.nlm.nih.gov/pubmed/23774290
Buckley M.T., Racimo F., Allentoft M.E., Jensen M.K., Jonsson A., Huang H., Hormozdiari F., Sikora M., Marnetto D., Eskin E., Jørgensen M.E., Grarup N., Pedersen O., Hansen T.,
Kraft P., Willerslev E., Nielsen R. 2017. Selection in Europeans on Fatty Acid Desaturases Associated with Dietary Changes. Molecular Biology and Evolution. 34(6): 1307–1318. https://doi.org/10.1093/molbev/msx103 https://www.ncbi.nlm.nih.gov/pubmed/28333262 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435082
Conde T.A., Neves B.F., Couto D., Melo T., Neves B., Costa M., Silva J., Domingues P., Domingues M.R. 2021. Microalgae as sustainable bio-factories of healthy lipids: Evaluating fatty acid content and antioxidant activity. Marine Drugs. 19(7): 357. https://doi.org/10.3390/md19070357 https://www.ncbi.nlm.nih.gov/pubmed/34201621 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307217
Couto D., Conde T.A., Melo T., Neves B., Costa M., Silva J., Domingues R., Domingues P. 2023. The chemodiversity of polar lipidomes of microalgae from different taxa. Algal Research. 70: 103006. https://doi.org/10.1016/j.algal.2023.103006
Couto D., Melo T., Conde T.A., Moreira A.S.P., Ferreira P., Costa M., Silva J., Domingues R., Domingues P. 2022. Food grade extraction of Chlorella vulgaris polar lipids: A comparative lipidomic study. Food Chemistry. 375: 131685. https://doi.org/10.1016/j.foodchem.2021.131685 https://www.ncbi.nlm.nih.gov/pubmed/34865930
Fernández-Sevilla J.M., Acién-Fernández F.G., Molina-Grima E. 2010. Biotechnological production of lutein and its applications. Applied Microbiology and Biotechnology. 86(1): 27–40. https://doi.org/10.1007/s00253-009-2420-y https://www.ncbi.nlm.nih.gov/pubmed/20091305
Fox J.M., Zimba P.V. 2018. Minerals and Trace Elements in Microalgae. In: Microalgae in Health and Disease Prevention. Cambridge: Acad. Press. Pp. 177–193. https://doi.org/10.1016/B978-0-12-811405-6.00008-6 https://www.ncbi.nlm.nih.gov/pubmed/29132648
Gornall A.G., Bardawill C.J., David M.M. 1949. Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry. 177(2): 751–766. https://doi.org/10.1016/S0021-9258(18)57021-6 https://www.ncbi.nlm.nih.gov/pubmed/18110453
Grobbelaar J.U. 2003. Quality control and assurance: crucial for the sustainability of the applied phycology industry. Journal of Applied Phycology. 15: 209–215. https://doi.org/10.1023/A:1023820711706
Harwood J.L., Okanenko A.A. 2003. Sulphoquinovosyl diacylglycerol (SQDG) - the sulpholipid of higher plants. In: Sulphur in Plants. Pp. 189–219. https://doi.org/10.1007/978-94-017-0289-8_11 https://www.ncbi.nlm.nih.gov/pubmed/28118817
Jacques F., Rippa S., Perrin Y. 2018. Physiology of L-carnitine in plants in light of the knowledge in animals and microorganisms. Plant Science. 274: 432–440. https://doi.org/10.1016/j.plantsci.2018.06.020 https://www.ncbi.nlm.nih.gov/pubmed/30080631
Jerney J., Spilling K. 2018. Large scale cultivation of microalgae: open and closed systems. Methods in Molecular Biology. 1980: 1–8. https://doi.org/10.1007/7651_2018_130 https://www.ncbi.nlm.nih.gov/pubmed/29480401
Kean E. 1968. A rapid sensitive spectrophotometric method for quantitative determination of sulfatides. Journal of Lipid Research. 9(3): 319–329. https://doi.org/10.1016/S0022-2275(20)43098-6 https://www.ncbi.nlm.nih.gov/pubmed/5646183
Lee Y.-K., Ding S.-Y. 1994. Cell cycle and accumulation of astaxanthin in Haematococcus lacustris (Chlorophyta). Journal of Phycology. 30(3): 445–449. https://doi.org/10.1111/j.0022-3646.1994.00445.x
Lee T.K., Nguyen T.T.H., Park N., Kwak S.H., Kim J., Jin S., Son G.M., Hur J., Choi J.I., Kim D. 2018. The use of fermented buckwheat to produce l‑carnitine enriched oyster mushroom. AMB Express. 8(1): 138. https://doi.org/10.1186/s13568-018-0664-6 https://www.ncbi.nlm.nih.gov/pubmed/30151668 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111021
Mendoza H., Freijanes K., Carmona L., Ramos A., Duarte V., Varela J., de la Jara A. 2008. Characterization of Dunaliella salina strains by flow cytometry: a new technique to select beta-carotene hyperproducing strains. Electronic Journal of Biotechnology. 11(4): 1–13. https://doi.org/10.2225/vol11-issue4-fulltext-2
Metsoviti M.N., Papapolymerou G., Karapanagiotidis I.T., Katsoulas N. 2020. Effect of light intensity and quality on growth rate and composition of Chlorella vulgaris. Plants. 9(1): 31. https://doi.org/10.3390/plants9010031 https://www.ncbi.nlm.nih.gov/pubmed/31878279 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020147
Mikhaylova S.V., Baydakova G.V., Boukina A.M., Boukina T.M., Shechter O.V., Ilina E.S., Zakharova E.Y. 2004. Combination of tandem mass spectrometry and lysosomal enzymes analysis-effective tool for selective screening for IEM in neurological clinic. Journal of Inherited Metabolic Disease. 27(1): 3. https://link.springer.com/article/10.1007/BF03356117
Mingyang M., Danni Y., Yue H., Park M., Gong Y., Hu Q. 2017. Effective control of Poterioochromonas malhamensis in pilot-scale culture of Chlorella sorokiniana GT-1 by maintaining CO2-mediated low culture pH. Algal Research. 26: 436–444. https://doi.org/10.1016/j.algal.2017.06.023
Mohandass R. 2019. Screening and characterization of high lipid accumulating microalga Ankistrodesmus sp. from freshwater environment. Іndian Journal of Experimental Biology. 57(12): 931–936.
Molino A., Lovine A., Casella P., Mehariya S., Chianese S., Cerbone A., Rimauro J., Musmarra D. 2018. Microalgae Characterization for Consolidated and New Application in Human Food, Animal Feed and Nutraceuticals. International Journal of Environmental Research and Public Health. 15(11): 2436. https://doi.org/10.3390/ijerph15112436 https://www.ncbi.nlm.nih.gov/pubmed/30388801 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266511
Mosibo O.K., Ferrentino G., Udenigwe C.C. 2024. Microalgae Proteins as sustainable ingredients in novel foods: Recent developments and challenges. Foods. 13(5): 733. https://doi.org/10.3390/foods13050733 https://www.ncbi.nlm.nih.gov/pubmed/38472846 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10930894
Mróz M., Parchem K., Jóźwik J., Domingues M.R., Kusznierewicz B. 2024. The Impact of Different Drying Methods on the Metabolomic and Lipidomic Profiles of Arthrospira platensis. Molecules. 29(8): 1747. https://doi.org/10.3390/molecules29081747 https://www.ncbi.nlm.nih.gov/pubmed/38675566 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11051859
Nova P., Martins A.P., Teixeira C., Abreu H., Silva J.G., Silva A.M., Freitas A.C., Gomes A.M. 2020. Foods with microalgae and seaweeds fostering consumers health: a review on scientific and market innovations. Journal of Applied Phycology. 32: 1789–1802. https://doi.org/10.1007/s10811-020-02129-w
Ovchynnikov Yu.A. 1974. New methods of analysis of amino acids, peptides and proteins. Мoscow: Mir. 154 p. [Овчинніков Ю.А. 1974. Нові методи аналізу амінокислот, пептидів та білків. М.: Mир. 154 с.] https://doi.org/10.1007/978-3-642-65905-8_11 https://www.ncbi.nlm.nih.gov/pubmed/28118817
Perez-Garcia O., de-Bashan L.E., Hernandez J.-P. & Bashan Y. 2010. Efficiency of growth and nutrient uptake from wastewater by heterotrophic, autotrophic, and mixotrophic cultivation of Chlorella vulgaris immobilized with Azospirillum brasilense. Journal of Phycology. 46(4): 800–812. https://doi.org/10.1111/j.1529-8817.2010.00862.x
Priyadarshani I., Rath B. 2012. Commercial and industrial applications of microalgae - a review. Journal of Algal Biomass Utilization. 3(4):89-100. https://doi.org/10.1263/jbb.101.87 https://www.ncbi.nlm.nih.gov/pubmed/16569602
Ru I.T.K., Sung Y.Y., Jusoh M., Wahid M.E.A., Nagappan T. 2020. Chlorella vulgaris: a perspective on its potential for combining high biomass with high value bioproducts. Applied Phycology. (1)1: 2–11. https://doi.org/10.1080/26388081.2020.1715256
Sakevich A.I., Klochenko P.D. 1998. Free amino acids in ecological metabolism of algae. Hydrobiological Journal. 34(6): 70–79. [Сакевич А.І., Клоченко П.Д. 1998. Вільні амінокислоти в екологічному метаболізмі водоростей. Гідробіологічний журнал. 34(6): 70–79.] https://doi.org/10.1615/HydrobJ.v34.i6.100
Santo G.E., Barros A., Costa M., Pereira H., Trovão M., Cardoso H., Carvalho B., Soares M., Correia N., Silva J.T., Mateus M., Silva J.L. 2023. Scenedesmus rubescens Heterotrophic Production Strategies for Added Value Biomass. Marine Drugs. 1(7): 411. https://doi.org/10.3390/md21070411 https://www.ncbi.nlm.nih.gov/pubmed/37504942 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381400
Sato N., Aoki M., Maru Y., Sonoike K., Minoda A., Tsuzuki M. 2003. Involvement of sulfoquinovosyl diacylglycerol in the structural integrity and heat-tolerance of photosystem II. Planta. 217(2): 245–251. https://doi.org/10.1007/s00425-003-0992-9 https://www.ncbi.nlm.nih.gov/pubmed/12783332
Shimojima M. 2011. Biosynthesis and functions of the plant sulfolipid. Progress in Lipid Research. 50(3): 234–239. https://doi.org/10.1016/j.plipres.2011.02.003 https://www.ncbi.nlm.nih.gov/pubmed/21371504
Siahbalaei R., Kavoosi G., Noroozi M. 2021. Manipulation of Chlorella vulgaris polyunsaturated ω-3 fatty acid profile by supplementation with vegetable amino acids and fatty acids. Phycological Research. 69(2): 116–123. https://doi.org/10.1111/pre.12449
Torres-Tiji Y., Fields F.J., Mayfield S.P. 2020. Microalgae as a future food source. Biotechnology Advances. 41:107536. https://doi.org/10.1016/j.biotechadv.2020.107536 https://www.ncbi.nlm.nih.gov/pubmed/32194145
Tsarenko P.M. 2011. Trebouxiophyceae. In: Algae of Ukraine: diversity, nomenclature, taxonomy, ecology and geography. Vol. 3. Chlorophyta. Eds P. Tsarenko, S. Wasser, E. Nevo. Ruggell: Gantner Verlag. Pp. 61–108.
Yongmanitchai W., Ward O.P. 1993. Positional distribution of fatty acids, and molecular species of polar lipids, in the diatom Phaeodactylum tricornutum. Journal of General Microbiology. 139(3): 465–472. https://doi.org/10.1099/00221287-139-3-465 https://www.ncbi.nlm.nih.gov/pubmed/20050416
Wang B., Chen M., Zheng M., Qiu Y. 2020. Responses of Two Coastal Algae (Skeletonema costatum and Chlorella vulgaris) to Changes in Light and Iron Levels. Journal of Phycology. 56(3): 618–629. https://doi.org/10.1111/jpy.12972 https://www.ncbi.nlm.nih.gov/pubmed/31965566
Whitney E., Rolfes S.R. 2018. Understanding Nutrition. Cengage Learning. 848 р.
Zolotareva O.K., Shnyukova E.I., Syvash O.O., Mykhailenko N.F. 2008. Prospects for the use of microalgae in biotechnology. Kyiv: Alterpress. 234 p. [Золотарьова О.К., Шнюкова Є.І., Сиваш О.О., Михайленко Н.Ф. 2008. Перспективи використання мікроводоростей у біотехнології. Київ: Альтерпрес. 234 с.]
