Paper deals with data on results of diatom study in water bodies of Ukrainian part of International Biosphere Reserve “Roztocze”. Ninety-six species of Bacillariophyta represented by 98 infraspecific taxa were revealed in periphyton of various water bodies of the reserve. Taxonomically they belong to three classes (Coscinodiscophyceae, Mediophyaceae and Bacillariophyceae), 5 subclasses (Coscinodiscophycidae, Talassiosirophycidae, Fragilariophy-cidae, Eunotiophycidae, Bacillariophycidae) 14 orders (Melosirales, Aulacoseirales, Thalassiosirales, Fragilariales, Tabellariales, Eunotiales, Mastogloiales, Cymbellales, Achnanthales, Naviculales, Thalassiophysales, Bacillariales, Rhopalodiales, Surirellales), 26 families and 48 genera. Their species diversity, taxonomic composition, details of occurrence in water bodies of various types, ecological and regional peculiarities are discussed. Three species are first cited for Ukraine: Sellaphora schadei (Krasske) C.E. Wetzel, L. Ector, B. Van de Vijver, Compère et D.G. Mann, Encyonopsis subminuta Krammer et E. Reichardt and Cymbella subturgidula Krammer. For noteworthy records original descriptions, micrographs and details of their sozological status are presented.
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