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Algologia 2019, 29(1): 30–39
https://doi.org/10.15407/alg29.01.030
Physiology, Biochemistry, Biophysics

Ethyl acetate and methanolic extracts from three algae and their potential antioxidant activity in vitro

Ebrahimzadeh M.A.1 , Khalili M.2 , Dehpour A.A.3
Abstract

Marine algae produce secondary metabolites with antioxidant activity and therapeutic effects. The aim of this study was to evaluate the antioxidant activity of ethyl acetate and methanolic extracts of three algal species: Nannochloropsis oculata (Droop) D.J. Hibberd, Enteromorpha sp., and Polysiphonia scopulorum Harvey. Ethyl acetate and methanolic extracts were prepared using the percolation method and evaluated for DPPH radical scavenging activity, nitric oxide scavenging activity, metal chelating activity, and reducing power activity. The means were compared using the Newman-Keuls Multiple Comparison test. The ethyl acetate extract of N. oculata was found to have the highest total phenol content (82.07 ± 1.32 mg gallic acid equivalent g-1 of extract). The maximum flavonoid content belonged to Enteromorpha sp. (74.79 ± 0.91 quercetin equivalent g-1 of extract). The maximum DPPH radical scavenging activity (67.73 ± 0.39%) belonged to ethyl acetate extract of P. scopulorum. The maximum nitric oxide scavenging activity and metal chelating activity belonged to ethyl acetate extract of N. oculata. The highest reducing power activity was observed in the methanolic extract of P. scopulorum. Ethyl acetate extracts of P. scopulorum and N. oculata show significant antioxidant activity, which may be due to their phenolic and flavonoid compounds.

Keywords: marine algae, Nannochloropsis oculata, Enteromorpha sp., Polysiphonia scopulorum, antioxidant activity

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References
  1. Agatonovic-Kustrin S., Morton D.W. 2017. Quantification of polyphenolic antioxidants and free radical scavengers in marine algae. J. Appl. Phycol. 30(1): 113–120. https://doi.org/10.1007/s10811-017-1139-x
  2. Cheung L.M., Cheung P.C.K., Ooi V.E.C. 2003. Antioxidant activity and total phenolics of edible mushroom extracts. Food. Chem. 81: 249–255. https://doi.org/10.1016/S0308-8146(02)00419-3
  3. Ebrahimzadeh M.A., Ebrahimzadeh M.K. 2014. Antioxidant activity of different fractions of Cantharellus cibarius methanolic extract. Int. J. Med. Mushrooms (accepted). https://www.ncbi.nlm.nih.gov/pubmed/25271862
  4. Ebrahimzadeh M.A., Khalili M., Dehpour A.A. 2018. Antioxidant activity of ethyl acetate and methanolic extracts of two marine algae, Nannochloropsis oculata and Gracilaria gracilis – an in vitro assay. Braz. J. Pharm. Sci. 54(1): e17280. https://doi.org/10.1590/s2175-97902018000117280
  5. Ebrahimzadeh M.A., Nabavi S.M., Nabavi S.F., Eslami S. 2010. Antioxidant and free radical scavenging activities of culinary-medicinal mushrooms, golden chanterelle Cantharellus cibarius and Angel's wings Pleurotus porrigens. Int. J. Med. Mushrooms. 12(3): 265–272. https://doi.org/10.1615/IntJMedMushr.v12.i3.50
  6. Ebrahimzadeh M.A., Safdari Y., Khalili M. 2015. Antioxidant activity of different fractions of methanolic extract of the golden chanterelle mushroom Cantharellus cibarius (higher basidiomycetes) from Iran. Int. J. Med. Mushrooms. 17(6): 557–565. https://doi.org/10.1615/IntJMedMushrooms.v17.i6.60 https://www.ncbi.nlm.nih.gov/pubmed/26349513
  7. Fernando I.S., Kim M., Son K.-T., Jeong Y., Jeon Y.-J. 2016. Antioxidant activity of marine algal polyphenolic compounds: a mechanistic approach. J. Med. Food. 19(7): 615–628. https://doi.org/10.1089/jmf.2016.3706 https://www.ncbi.nlm.nih.gov/pubmed/27332715
  8. Jiménez-Escrig A., Jiménez-Jiménez I., Pulido R., Saura-Calixto F. 2001. Antioxidant activity of fresh and processed edible seaweeds. J. Sci. Food. Agric. 81(5): 530–534. https://doi.org/10.1002/jsfa.842
  9. Khalili M., Ebrahimzadeh M.A. 2015. A review on antioxidants and some of their common evaluation methods. J. Mazandaran Univ. Med. Sci. 24(120): 188–208.
  10. Khalili M., Ebrahimzadeh M.A., Kosaryan M., Abbasi A., Azadbakht M. 2015. Iron chelation and liver disease healing activity of edible mushroom (Cantharellus cibarius), in vitro and in vivo assays. RSC Adv. 5(7): 4804–4810. https://doi.org/10.1039/C4RA11561A
  11. Lim S., Cheung P., Ooi V., Ang P. 2002. Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum. J. Agr. Food. Chem. 50(13): 3862–3866. https://doi.org/10.1021/jf020096b https://www.ncbi.nlm.nih.gov/pubmed/12059172
  12. Mozdastan S., Ebrahimzadeh M.A., Khalili M. 2015. Comparing the impact of different extraction methods on antioxidant activities of myrtle (Myrtus communis L.). J. Mazandaran Univ. Med. Sci. 25(127): 10–24.
  13. Saeed N., Khan M.R., Shabbir M. 2012. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement. and Altern. Med. 12: 221. https://doi.org/10.1186/1472-6882-12-221 https://www.ncbi.nlm.nih.gov/pubmed/23153304 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524761
  14. Sameeh M.Y., Mohamed A.A., Elazzazy A.M. 2016. Polyphenolic contents and antimicrobial activity of different extracts of Padina boryana Thivy and Enteromorpha sp. marine algae. J. Appl. Pharm. Sci. 6(9): 87–92. https://doi.org/10.7324/JAPS.2016.60913
  15. Shon M.-Y., Choi S.-D., Kahng G.-G., Nam S.-H., Sung N.-J. 2004. Antimutagenic, antioxidant and free radical scavenging activity of ethyl acetate extracts from white, yellow and red onions. Food. Chem. Toxicol. 42(4): 659–666. https://doi.org/10.1016/j.fct.2003.12.002 https://www.ncbi.nlm.nih.gov/pubmed/15019191
  16. Urquiaga I., Leighton F. 2000. Plant polyphenol antioxidants and oxidative stress. Biol. Res. 33(2): 55–64. https://doi.org/10.4067/S0716-97602000000200004 https://www.ncbi.nlm.nih.gov/pubmed/15693271