ISSN (print) 0868-8540, (online) 2413-5984
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Algologia 2021, 31(2): 170–199
Applied Algology

Phyto-pharmacological investigation of algae Kappaphycus alvarezii (Doty) Doty ex Silva for oral diseases (Rhodophyta)

Sharan L.V., Vennila J.J.

Oral infections (gingivitis and periodontitis) and oral cancer are under rise in developing countries. Products with antibacterial and antioxidant activity can provide a combined approach to treat oral disorders. Marine algae is a reservoir of rich bioactive phytochemicals and are considered to be potential candidates in natural pharmaceutics. Kappaphycus alvarezii is a marine algae widely cultivated for food applications. The current study investigates the phyto-pharmacological properties of K. alvarezii for oral diseases. Different polarity solvents (ethanol, ethyl acetate and chloroform) were used in the extraction of bioactive components of K. alvarezii, partially characterized by GCMS and studied for their antioxidant, antimicrobial and cytotoxic activity. All the K. alvarezii extracts exhibited good antioxidant activity and potential efficacy against oral pathogenic microbes. Although K. alvarezii extracts were found to be safe for normal Vero cells, their inhibitory activity on oral cancer cells (KB-3-1 cell lines) was found to be low. These findings have suggested the possibility of K. alvarezii using in the dental preparation/product to combat oral infections.

Keywords: Kappaphycus alvarezii, marine algae, oral disease, dental applications, periodontitis, antioxidant, antimicrobial and cytotoxic activity

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  1. Akwu N.A., Naidoo Y., Singh M., Nundkumar N., Lin J. 2019. Phytochemical screening, in vitro evaluation of the antimicrobial, antioxidant and cytotoxicity potentials of Grewia lasiocarpa E.Mey. ex Harv. South Afr. J. Bot. 123: 180–192.
  2. Andreadis C., Vahtsevanos K., Sidiras T., Thomaidis I., Antoniadis K., Mouratidou D. 2003. 5-Fluorouracil and cisplatin in the treatment of advanced oral cancer. Oral Oncol. 39: 380–385.
  3. Ariffin F.D., Abdullah A., Meng C.K., Ariffin S.H., Sahani M. 2014. Cytotoxic effect of red seaweeds Kappaphycus alvarezii and Kappaphycus striatum on hepatocarcinoma HepG2 cell line. Adv. Environ. Biol. 8(15): 79–84.
  4. Balasubramaniam V., Mustar S., Mustafa K.N., Abd R.A., Mohd N.M.F., Wilcox M.D., Chater P.I., Brownlee I.A., Pearson J.P. 2013. Inhibitory activities of three Malaysian edible seaweeds on lipase and α-amylase. J. Appl. Phycol. 25: 1405–1412.
  5. Banu K., Cathrine L. 2015. General techniques involved in phytochemical analysis. Int. J. Adv. Res. Chem. Sci. 2: 25–32.
  6. Beulah G.G.P., Tresina Soris P., Mohan V.R. 2018. GC-MS Determination of Bioactive Compounds of Dendrophthoe falcata (L.F) Ettingsh: An Epiphytic Plant. Int J Health Sci Res. 8: 261–269.
  7. Campos F.F., Ramos J.P., De Oliveira D.M., Alves T.M., De Souza-Fagundes E.M., Zani C.L., Sampaio F.C., Converti A., Cota B.B. 2017. In vitro leishmanicidal, antibacterial and antitumour potential of anhydrocochlioquinone A obtained from the fungus Cochliobolus sp., J. Biosci. 42(4): 657–664.
  8. Chang V.S., Okechukwu P.N., Teo S., Sen. 2017. The properties of red seaweed (Kappaphycus alvarezii) and its effect on mammary carcinogenesis. Biomed. Pharm. 87: 296–301.
  9. Chaula D., Laswai H., Chove B., Dalsgaard A., Mdegela R., Jacobsen C., Hyldig G. 2019. Effect of clove (Syzygium aromaticum) and seaweed (Kappaphycus alvarezii) water extracts pretreatment on lipid oxidation in sun-dried sardines (Rastrineobola argentea) from Lake Victoria, Tanzania. Food Sci. Nutr. 7: 1406–1416.
  10. Cherian C., Vennila J.J., Sharan L. 2019. Marine bromophenols as an effective inhibitor of virulent proteins (peptidyl arginine deiminase, gingipain R and hemagglutinin A) in Porphyromas gingivalis, Arch. Oral Biol. 100: 119–128.
  11. Chinsembu K.C. 2016. Plants and other natural products used in the management of oral infections and improvement of oral health. Acta Trop. 154: 6–18.
  12. Cory H., Passarelli S., Szeto J., Tamez M., Mattei J. 2018. The Role of Polyphenols in Human Health and Food Systems: A Mini-Review. Front. Nutr. 5: 1–9.
  13. Cyriac B., Eswaran K. 2016. Anti- hyperglycemic effect of aqueous extract of Kappaphycus alvarezii (Doty) doty ex. p. silva in alloxan-induced diabetic rats. J. Appl. Phycol. 28: 2507–2513.
  14. Dai Chun-Ling, Yong-Ju, Liang, Yan-Sheng Wang, Amit K.T., Yan-Yan Yan, Fang Wang, Zhe-Sheng Chen, Xiu-Zhen Tong, Li-Wu Fu. 2009. Sensitization of ABCG2-overexpressing cells to conventional chemotherapeutic agent by sunitinib was associated with inhibiting the function of ABCG2. Cancer letters. 279: 74–83.
  15. Daniluk T., Tokajuk G., Cylwik-Rokicka D., Rozkiewicz D., Zaremba M.L., Stokowska W. 2006. Aerobic and anaerobic bacteria in subgingival and supragingival plaques of adult patients with periodontal disease. Adv. Med. Sci. 51: 81–85.
  16. El Gamal A.A. 2011. Biological Importance of Marine Algae. Handbook of Marine Macroalgae. Saudi Pharm. J. 18: 1–35.
  17. Elkady A.I., Ramadan W.S. 2016. The aqueous extract of cinnamon bark ameliorated cisplatin-induced cytotoxicity in vero cells without compromising the anticancer efficiency of cisplatin. Biomed. Papers J. Palacký Univ. Olomouc, Faculty of Medicine and Dentistry, Olomouc, Czech Republic. 160: 363–371.
  18. Ganesh S., Vennila J.J. 2011. Phytochemical analysis of Acanthus ilicifolius and Avicennia officinalis by GC-MS. Res. J. Phytochem. 5(1): 60–65.
  19. Godha S., Dasar P.L., Sandesh N., Mishra P., Kumar S., Balsaraf S., Bhadauria U.S., Vyas S. 2015. Oral Health: A Window to your Overall Health. Int. J. Oral Health Med. Res. 2: 105–108.
  20. Jennifer N., Kiruba M., Pradeep M.A., Jemima S., Juliana B. 2015. Study of Phytoconstituents and antibacterial activity of Kappaphycus alvarezii. Int. J. Cur. Microbiol. App. Sci.4: 1209–1217.
  21. Kanatt S.R., Lahare P., Chawla S.P., Sharma A. 2016. Kappaphycus alvarezii: Its antioxidant potential and use in bioactive packaging films. J. Microbiol. Biotechnol. Food Sci. 5: 1–6.
  22. Kiruba N.J., Pradeep M.A., Thatheyus A.J. 2018. Discovering Promising Anti-cancer Drug Candidates from Marine Algae. Sci. Int. 6(2): 44–50.
  23. Kumar U.S., Jothy S.L., Kavitha N., Chen Y., Kanwar J.R., Sasidharan S. 2018. Genoprotection and Cytotoxicity of Cassia surattensis Seed Extract on Vero Cell Evaluated by Comet and Cytotoxicity Assays. Proc. Natl. Acad. Sci., India, Sect. B: Biol. Sci. 88: 313–320.
  24. Lalopua V.P., Purnomo H., Sukoso, Aulani'am. 2011. Red seaweed (Kappaphycus alvarezii DOTY) from Mollucas island water as potential flavonoid resource of natural antioxidant, Livest. Res. Rural Dev. 23: 254.
  25. Lara N.L., van den Driesche S., Macpherson S., França L.R., Sharpe R.M. 2017. Dibutyl phthalate induced testicular dysgenesis originates after seminiferous cord formation in rats. Sci. Rep. 7(1): 1–3.
  26. Lau T.Y., Vittal D.F., Chew C.S.Y., Yong W.T.L. 2014. Antiproliferative Potential of Extracts from Kappaphycus Seaweeds on HeLa Cancer Cell Lines. Sains Malays. 43: 1895–1900.
  27. Ling A.L.M., Yasir S., Matanjun P., Abu Bakar M.F. 2015. Effect of different drying techniques on the phytochemical content and antioxidant activity of Kappaphycus alvarezii. J. Appl. Phycol. 27: 1717–1723.
  28. Miazek K., Kratky L., Sulc R., Jirout T., Aguedo M., Richel A., Goffin D. 2017. Effect of organic solvents on microalgae growth, metabolism and industrial bioproduct extraction: a review. Int. J Mol. Sci. 18(7): 1429.
  29. Muhaidat R., Al-Qudah M.A., Samir O., Jacob J.H., Hussein E., Al-Tarawneh I.N., Bsoul E., Abu Orabi S.T. 2015. Phytochemical investigation and in vitro antibacterial activity of essential oils from Cleome droserifolia (Forssk.) Delile and C. trinervia Fresen. (Cleomaceae). South Afr. J. Bot. 99: 21–28.
  30. Munir N., Sharif N., Naz S., Farkhanda M. 2013. Algae: A potent antioxidant source. Sky J. Microbiol. Res.1: 22–31.
  31. Nagarani N., Kumaraguru A.K. 2012. Investigation of the effect of K. alvarezii on antioxidant enzymes, cell viability and DNA damage in male rats. Front Life Sci. 6: 97–105.
  32. Pradeep C.K., Channarayapatna-Ramesh S., Kujur S., Basavaraj G.L., Madhusudhan M.C., Udayashankar A.C. 2018. Evaluation of in vitro Antioxidant Potential of Phyllanthus acidus Fruit. Res. J. Life Sci., Bioinfo., Pharm. and Chem. Sci. 4: 30–41.
  33. Pramitha V.S., Sree Kumari N. 2016. Anti-inflammatory, antioxidant, phytochemical and GC-MS analysis of marine brown macroalga, Sargassum wightii. Int. J. Pharm. Chem. Biol. Sci. 6(1): 7–15.
  34. Rajesh K.D., Vasantha S., Panneerselvam A., Valsala R.N., Jeyathilakan N. 2016. Phytochemical Analysis, in vitro Antioxidant Potential and Gas Chromatography-Mass Spectrometry Studies of Dicranopteris Linearis, Asian J. Pharm. Clin. Res. 9(2): 1–6.
  35. Ramamoorthy S., Gnanakan A., Lakshmana S., Meivelu M., Jeganathan A. 2018. Structural characterization and anticancer activity of extracellular polysaccharides from ascidian symbiotic bacterium Bacillus thuringiensis, Carbohydr. Polym. 190: 113–120.
  36. Rasmussen L.M., Sen N., Vera J.C., Liu X., Craig Z.R. 2017. Effects of in vitro exposure to dibutyl phthalate, mono-butyl phthalate, and acetyl tributyl citrate on ovarian antral follicle growth and viability. Biol. Reprod. 96(5): 1105–1117.
  37. San S.M., Opperman L.A., Allen E.P., Svoboda K.K. 2011. Use of antioxidants in oral healthcare. Compendium of continuing education in dentistry (Jamesburg, NJ: 1995). 32(9): E156–159.
  38. Seetharaman S., Indra V., Selva Muthu B., Daisy A., Geetha S. 2016. Phytochemical profiling and Antibacterial potential of Kappaphycus alvarezii methanol extract against clinical isolated bacteria. World J. Pharm. Pharmctol. Sci. 5(6): 1328–1337.
  39. Seleem D., Pardi V., Murata R.M. 2017. Review of flavonoids: A diverse group of natural compounds with anti-Candida albicans activity in vitro. Arch. Oral Biol. 76: 76–83.
  40. Shameel M., Afaq-Husain S., Zarina A. 2013. Phycochemical studies on seven species of Rhodophycota from Karachi coast of Pakistan. Int. J. Algae. 15(3): 285–290.
  41. Sharan L., Vennila J. 2019. Marine algae as a promising natural resource in treating Periodontitis: Current status and applications. Res. J. Biotechnol. 14: 71–82.
  42. Singleton V.L., Orthofer R., Lamuela-Raventos R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Methods Enzymol. 299: 152–178.
  43. Sumayya S., Murugan K. 2017. Phytochemical screening, RP-HPLC and FTIR Analysis of Kappaphycus alvarezii (Doty) Doty ex P.C.Silva: Macro red algae. J. Pharmacogn. Phytochem. 6: 325–330.
  44. Sumayya S., Murugan K. 2018. Fractionationation of purified terpenoids from red algae Hypnea musciformis (Wulfen) J.V.Lamouroux. and Kappaphycus alvarezii (Doty) Doty ex P.C. Silva. By Gc: Ms analysis. J. Pharm. Phytochem. 7: 636–640.
  45. Tripathi P., Vikram B., Preeti U., Manika J., Shweta G., Sadaf N. 2019. Antioxidant therapy (lycopene and green tea extract) in periodontal disease: A promising paradigm, J. Indian Soc. Periodontol. 23: 25.
  46. Veneziani R.C.S., Ambrósio S.R., Martins C.H.G., Lemes D.C., Oliveira L.C. 2017. Chap. 4 - Antibacterial Potential of Diterpenoids. Stud. Nat. Prod. Chem. 54: 109–139.–5.00004-8
  47. Vyas T., Sood P., Kaur M. 2018. Antioxidants in Oral Diseases and Future Prospects and their Application in Dentistry. J. Adv. Med. Dent. Sci. Res. 6: 53–62.
  48. West K., Crawford A. 2016. Marine Bio discovery Goes Deeper: Using In. Vivo Bioassays Based on Model Organisms to Identify Biomedically Relevant Marine Metabolites. Planta Med. 82: 754–760.
  49. Yamuna P., Abirami P., Vijayashalini P., Sharmila M. 2017. GC-MS analysis of bioactive compounds in the entire plant parts of ethanolic extract of Gomphrena decumbens Jacq. J. Med. Plants Stud. 5(3): 31–37.
  50. Zhou Y., Zhang W., Liu X., Yu H., Lu X., Jiao B. 2017. Inhibitors of Protein Tyrosine Phosphatase 1B from Marine Natural Products. Chem. Biodivers. 14: e1600462.
  51. Zolotareva E.K., Mokrosnop V.M., Stepanov S.S. 2019. Polyphenol Compounds of Macroscopic and Microscopic Algae. Int. J. Algae. 21(1): 5–24.